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recombinant murine egf  (Thermo Fisher)


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    Thermo Fisher recombinant murine egf
    Recombinant Murine Egf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+murine+egf/recombinant+murine+egf/pm40243204-108-70-74
    Average 90 stars, based on 1 article reviews
    recombinant murine egf - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Tritrichomonas muris sensitizes the intestinal epithelium to doxorubicin-induced apoptosis.
    Article Snippet: Crypts were resuspended in 80% phenol red-free, growth factor-reduced Matrigel (Corning; 223 356231) and plated as 30 μL droplets in 48-well plates. .. Matrigel was allowed to polymerize at RT for 5 224 min, then at 37°C for 15 min. ENR media was overlaid at 200 μL per well: Advanced DMEM/F12, 1X 225 N2 (Thermo Fisher; 17502048), 1X B27 w/o vitamin A (Thermo Fisher; 12587010), 1X HEPES (Gibco; 226 15630080), 1X Penicillin/Streptomycin (Sigma-Aldrich; P4333-100ML), 1X Glutamax (Gibco; 227 35050061), 10% RSPO1-CM (made using RSPO1 transfected HEK293T cells following manufacturer 228 protocol; Sigma Aldrich; SCC111), 50ng/mLrecombinant murine EGF (Gibco; PMG8041), and 229 100ng/mL recombinant human NOGGIN (PeproTech; 120-10C-20UG). .. 500μg/mL Primocin (Invitrogen; 230 ant-pm-1) and 10mM Y27632 (Selleck Chemicals; S1049) were added to overlay media for the first 48hr 231 after plating crypts and then excluded from all other media changes.

    Recombinant:

    Article Title: Tritrichomonas muris sensitizes the intestinal epithelium to doxorubicin-induced apoptosis.
    Article Snippet: Crypts were resuspended in 80% phenol red-free, growth factor-reduced Matrigel (Corning; 223 356231) and plated as 30 μL droplets in 48-well plates. .. Matrigel was allowed to polymerize at RT for 5 224 min, then at 37°C for 15 min. ENR media was overlaid at 200 μL per well: Advanced DMEM/F12, 1X 225 N2 (Thermo Fisher; 17502048), 1X B27 w/o vitamin A (Thermo Fisher; 12587010), 1X HEPES (Gibco; 226 15630080), 1X Penicillin/Streptomycin (Sigma-Aldrich; P4333-100ML), 1X Glutamax (Gibco; 227 35050061), 10% RSPO1-CM (made using RSPO1 transfected HEK293T cells following manufacturer 228 protocol; Sigma Aldrich; SCC111), 50ng/mLrecombinant murine EGF (Gibco; PMG8041), and 229 100ng/mL recombinant human NOGGIN (PeproTech; 120-10C-20UG). .. 500μg/mL Primocin (Invitrogen; 230 ant-pm-1) and 10mM Y27632 (Selleck Chemicals; S1049) were added to overlay media for the first 48hr 231 after plating crypts and then excluded from all other media changes.

    Article Title: Sox9 EGFP Defines Biliary Epithelial Heterogeneity Downstream of Yap Activity
    Article Snippet: .. Organoids grown in TNFα conditions were overlaid with TNFα media (Advanced DMEM/F12 [Gibco], B27 supplement without vitamin A [Thermo Fisher], N2 supplement [Thermo Fisher], Glutamax [Gibco], 10 mmol/L HEPES [Gibco], penicillin/streptomycin [Gibco], 25 ng/mL recombinant murine EGF [Gibco], 50 ng/mL recombinant human HGF [Peprotech], 10 μmol/L Y-27632 [Selleck Chemicals], 1 μmol/L A8301 [Tocris; 2939], 3 μmol/L CHIR99021 [Selleck Chemicals; S1263], and 100 ng/mL recombinant murine TNFα [Peprotech; 315-01A]. ..

    Article Title: A novelty co-culture system for Ulcerative Colitis modeling and drug discovery
    Article Snippet: The crypt pellet was resuspended in 30% Matrigel (Corning 356231) in 24-well plate and incubated at 37°C for 15 min to allow Matrigel to polymerize. .. 0.5 mL complete medium containing 10% FBS, 1% Pen/Strep (Quality Biological 120095721), 1%L-glutamine (Gibco 35050061), 1% sodium pyruvate (Gibco 11360), 2% non-essential amino acids (Gibco 11130), 2.5% 1 M HEPES (Quality Biological 118089721), 50μM 2-mercaptoethanol (Sigma M6250), 50 ng/ml recombinant murine EGF (Invitrogen PMG8041),100 ng/ml recombinant murine Noggin (Peprotech 250–38),500 ng/ml recombinant human R-spondin (R&D Systems4645-RS) were added to each well. ..

    Article Title: Inhibition of RON kinase potentiates anti-CTLA-4 immunotherapy to shrink breast tumors and prevent metastatic outgrowth
    Article Snippet: .. Tumor cells were cultured in DME:F12 medium (Gibco, Invitrogen) supplemented with fetal bovine serum (10%) (Gibco, Invitrogen), insulin-transferrin-selenium-ethanolamine (1x) (Gibco, Invitrogen), recombinant murine EGF (10 ng/ml) (Invitrogen), hydrocortisone (1 μg/ml) (Sigma), penicillin-streptomycin-gentamycin (Gibco, Invitrogen) (1x) for a maximum of 4 days using previously described methods , prior to injection into mice. .. MC38 colon carcinoma cells were purchased from Kerafast (Boston, USA), and maintained in Dulbecco’s Modified Eagle Medium (DMEM) containing high glucose and GlutaMAX (Gibco), supplemented with heat-inactivated fetal bovine serum (10%), nonessential amino acids (0.1 mM), sodium pyruvate (1 mM), penicillin/streptomycin (100 U/ml), gentamycin (50 mg/ml) and HEPES (10 mM).

    Article Title: Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer
    Article Snippet: Digests were centrifuged and resuspended in 100% Matrigel (Corning #356231). .. Cells were cultured in complete mouse organoid feed media (30) containing Advanced DMEM/F-12 (Thermo Fisher Scientific #12634028), 1 1M HEPES (diluted to 10 mmol/L; Gibco, #15630056), 1X penicillin/streptomycin (100X stock; Thermo Fisher Scientific #15140122), 1XGlutaMAXSupplement (Gibco, #35050061), 0.5 mmol/L (0.21 mg/mL) A83–01 (TGFb inhibitor; Tocris, #2939), recombinant murine EGF (mEGF; 0.05 mg/mL; Gibco, #PMG8041), recombinant FGF (FGF-10; 0.1 mg/mL; Peprotech, #100–26–250 mg), 0.01 mmol/L (0.021 mg/mL) Gastrin I (Tocris, #3006), 0.1 mg/mL, recombinant mNoggin, 1.25 mmol/L (0.2 mg/mL; peprotech #250– 38–250ug), N-acetylcysteine 10mmol/L (1.22mg/mL; Sigma, #A9165– 5g), nicotinamide 10 mmol/L (Sigma, #N0636–100G), 1X B27 supplement (Thermo Fisher Scientific, #17504044), 10% R-spondinconditioned media (produced in-house from a cell line expressing recombinant R-spondin: Trevigen Cat # 3710–001–01) based on the method described in ref. (30), Y-27632 10.5 mmol/L (3.38 mg/ mL; Rho kinase inhibitor; Sigma, #Y0503–5 mg). ..

    Article Title: Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer
    Article Snippet: Digests were centrifuged and resuspended in 100% Matrigel (Corning #356231). .. Cells were cultured in complete mouse organoid feed media ( ) containing Advanced DMEM/F-12 (Thermo Fisher Scientific #12634028), 1×1M HEPES (diluted to 10 mmol/L; Gibco, #15630056), 1X penicillin/streptomycin (100X stock; Thermo Fisher Scientific #15140122), 1X GlutaMAX Supplement (Gibco, #35050061), 0.5 μmol/L (0.21 μg/mL) A83–01 (TGFβ inhibitor; Tocris, #2939), recombinant murine EGF (mEGF; 0.05 μg/mL; Gibco, #PMG8041), recombinant FGF (FGF-10; 0.1 μg/mL; Peprotech, #100–26–250 μg), 0.01 μmol/L (0.021 μg/mL) Gastrin I (Tocris, #3006), 0.1 μg/mL, recombinant mNoggin, 1.25 mmol/L (0.2 mg/mL; peprotech #250–38–250ug), N-acetylcysteine 10 mmol/L (1.22 mg/mL; Sigma, #A9165–5g), nicotinamide 10 mmol/L (Sigma, #N0636–100G), 1X B27 supplement (Thermo Fisher Scientific, #17504044), 10% R-spondin-conditioned media (produced in-house from a cell line expressing recombinant R-spondin: Trevigen Cat # 3710–001–01) based on the method described in ref. , Y-27632 10.5 μmol/L (3.38 μg/mL; Rho kinase inhibitor; Sigma, #Y0503–5 mg). ..

    Article Title: Sox9 EGFP Defines Biliary Epithelial Heterogeneity Downstream of Yap Activity
    Article Snippet: .. After polymerization, droplets were overlaid with 200 μL Biliary Expansion Media (50% Advanced DMEM/F12 [Gibco], 40% WNT3A-conditioned media, 10% RSPO1-conditioned media, B27 supplement without vitamin A [Thermo Fisher], N2 supplement [Thermo Fisher], Glutamax [Gibco], 10 mmol/L HEPES [Gibco], penicillin/streptomycin [Gibco], 50 ng/mL recombinant murine EGF [Gibco; PMG8043], 100 ng/mL recombinant human Noggin [Peprotech, Rocky Hill, NJ; 120-10C], 100 ng/mL recombinant human FGF10 [Peprotech; 100-26], 10 μmol/L recombinant human gastrin [Sigma-Aldrich; G9145], 50 ng/mL recombinant human HGF [Peprotech 100-39H], 10 mmol/L nicotinamide, and 10 μmol/L Y-27632 [Selleck Chemicals]). ..

    Cell Culture:

    Article Title: Inhibition of RON kinase potentiates anti-CTLA-4 immunotherapy to shrink breast tumors and prevent metastatic outgrowth
    Article Snippet: .. Tumor cells were cultured in DME:F12 medium (Gibco, Invitrogen) supplemented with fetal bovine serum (10%) (Gibco, Invitrogen), insulin-transferrin-selenium-ethanolamine (1x) (Gibco, Invitrogen), recombinant murine EGF (10 ng/ml) (Invitrogen), hydrocortisone (1 μg/ml) (Sigma), penicillin-streptomycin-gentamycin (Gibco, Invitrogen) (1x) for a maximum of 4 days using previously described methods , prior to injection into mice. .. MC38 colon carcinoma cells were purchased from Kerafast (Boston, USA), and maintained in Dulbecco’s Modified Eagle Medium (DMEM) containing high glucose and GlutaMAX (Gibco), supplemented with heat-inactivated fetal bovine serum (10%), nonessential amino acids (0.1 mM), sodium pyruvate (1 mM), penicillin/streptomycin (100 U/ml), gentamycin (50 mg/ml) and HEPES (10 mM).

    Article Title: Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer
    Article Snippet: Digests were centrifuged and resuspended in 100% Matrigel (Corning #356231). .. Cells were cultured in complete mouse organoid feed media (30) containing Advanced DMEM/F-12 (Thermo Fisher Scientific #12634028), 1 1M HEPES (diluted to 10 mmol/L; Gibco, #15630056), 1X penicillin/streptomycin (100X stock; Thermo Fisher Scientific #15140122), 1XGlutaMAXSupplement (Gibco, #35050061), 0.5 mmol/L (0.21 mg/mL) A83–01 (TGFb inhibitor; Tocris, #2939), recombinant murine EGF (mEGF; 0.05 mg/mL; Gibco, #PMG8041), recombinant FGF (FGF-10; 0.1 mg/mL; Peprotech, #100–26–250 mg), 0.01 mmol/L (0.021 mg/mL) Gastrin I (Tocris, #3006), 0.1 mg/mL, recombinant mNoggin, 1.25 mmol/L (0.2 mg/mL; peprotech #250– 38–250ug), N-acetylcysteine 10mmol/L (1.22mg/mL; Sigma, #A9165– 5g), nicotinamide 10 mmol/L (Sigma, #N0636–100G), 1X B27 supplement (Thermo Fisher Scientific, #17504044), 10% R-spondinconditioned media (produced in-house from a cell line expressing recombinant R-spondin: Trevigen Cat # 3710–001–01) based on the method described in ref. (30), Y-27632 10.5 mmol/L (3.38 mg/ mL; Rho kinase inhibitor; Sigma, #Y0503–5 mg). ..

    Article Title: Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer
    Article Snippet: Digests were centrifuged and resuspended in 100% Matrigel (Corning #356231). .. Cells were cultured in complete mouse organoid feed media ( ) containing Advanced DMEM/F-12 (Thermo Fisher Scientific #12634028), 1×1M HEPES (diluted to 10 mmol/L; Gibco, #15630056), 1X penicillin/streptomycin (100X stock; Thermo Fisher Scientific #15140122), 1X GlutaMAX Supplement (Gibco, #35050061), 0.5 μmol/L (0.21 μg/mL) A83–01 (TGFβ inhibitor; Tocris, #2939), recombinant murine EGF (mEGF; 0.05 μg/mL; Gibco, #PMG8041), recombinant FGF (FGF-10; 0.1 μg/mL; Peprotech, #100–26–250 μg), 0.01 μmol/L (0.021 μg/mL) Gastrin I (Tocris, #3006), 0.1 μg/mL, recombinant mNoggin, 1.25 mmol/L (0.2 mg/mL; peprotech #250–38–250ug), N-acetylcysteine 10 mmol/L (1.22 mg/mL; Sigma, #A9165–5g), nicotinamide 10 mmol/L (Sigma, #N0636–100G), 1X B27 supplement (Thermo Fisher Scientific, #17504044), 10% R-spondin-conditioned media (produced in-house from a cell line expressing recombinant R-spondin: Trevigen Cat # 3710–001–01) based on the method described in ref. , Y-27632 10.5 μmol/L (3.38 μg/mL; Rho kinase inhibitor; Sigma, #Y0503–5 mg). ..

    Injection:

    Article Title: Inhibition of RON kinase potentiates anti-CTLA-4 immunotherapy to shrink breast tumors and prevent metastatic outgrowth
    Article Snippet: .. Tumor cells were cultured in DME:F12 medium (Gibco, Invitrogen) supplemented with fetal bovine serum (10%) (Gibco, Invitrogen), insulin-transferrin-selenium-ethanolamine (1x) (Gibco, Invitrogen), recombinant murine EGF (10 ng/ml) (Invitrogen), hydrocortisone (1 μg/ml) (Sigma), penicillin-streptomycin-gentamycin (Gibco, Invitrogen) (1x) for a maximum of 4 days using previously described methods , prior to injection into mice. .. MC38 colon carcinoma cells were purchased from Kerafast (Boston, USA), and maintained in Dulbecco’s Modified Eagle Medium (DMEM) containing high glucose and GlutaMAX (Gibco), supplemented with heat-inactivated fetal bovine serum (10%), nonessential amino acids (0.1 mM), sodium pyruvate (1 mM), penicillin/streptomycin (100 U/ml), gentamycin (50 mg/ml) and HEPES (10 mM).

    other:


    Produced:

    Article Title: Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer
    Article Snippet: Digests were centrifuged and resuspended in 100% Matrigel (Corning #356231). .. Cells were cultured in complete mouse organoid feed media (30) containing Advanced DMEM/F-12 (Thermo Fisher Scientific #12634028), 1 1M HEPES (diluted to 10 mmol/L; Gibco, #15630056), 1X penicillin/streptomycin (100X stock; Thermo Fisher Scientific #15140122), 1XGlutaMAXSupplement (Gibco, #35050061), 0.5 mmol/L (0.21 mg/mL) A83–01 (TGFb inhibitor; Tocris, #2939), recombinant murine EGF (mEGF; 0.05 mg/mL; Gibco, #PMG8041), recombinant FGF (FGF-10; 0.1 mg/mL; Peprotech, #100–26–250 mg), 0.01 mmol/L (0.021 mg/mL) Gastrin I (Tocris, #3006), 0.1 mg/mL, recombinant mNoggin, 1.25 mmol/L (0.2 mg/mL; peprotech #250– 38–250ug), N-acetylcysteine 10mmol/L (1.22mg/mL; Sigma, #A9165– 5g), nicotinamide 10 mmol/L (Sigma, #N0636–100G), 1X B27 supplement (Thermo Fisher Scientific, #17504044), 10% R-spondinconditioned media (produced in-house from a cell line expressing recombinant R-spondin: Trevigen Cat # 3710–001–01) based on the method described in ref. (30), Y-27632 10.5 mmol/L (3.38 mg/ mL; Rho kinase inhibitor; Sigma, #Y0503–5 mg). ..

    Article Title: Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer
    Article Snippet: Digests were centrifuged and resuspended in 100% Matrigel (Corning #356231). .. Cells were cultured in complete mouse organoid feed media ( ) containing Advanced DMEM/F-12 (Thermo Fisher Scientific #12634028), 1×1M HEPES (diluted to 10 mmol/L; Gibco, #15630056), 1X penicillin/streptomycin (100X stock; Thermo Fisher Scientific #15140122), 1X GlutaMAX Supplement (Gibco, #35050061), 0.5 μmol/L (0.21 μg/mL) A83–01 (TGFβ inhibitor; Tocris, #2939), recombinant murine EGF (mEGF; 0.05 μg/mL; Gibco, #PMG8041), recombinant FGF (FGF-10; 0.1 μg/mL; Peprotech, #100–26–250 μg), 0.01 μmol/L (0.021 μg/mL) Gastrin I (Tocris, #3006), 0.1 μg/mL, recombinant mNoggin, 1.25 mmol/L (0.2 mg/mL; peprotech #250–38–250ug), N-acetylcysteine 10 mmol/L (1.22 mg/mL; Sigma, #A9165–5g), nicotinamide 10 mmol/L (Sigma, #N0636–100G), 1X B27 supplement (Thermo Fisher Scientific, #17504044), 10% R-spondin-conditioned media (produced in-house from a cell line expressing recombinant R-spondin: Trevigen Cat # 3710–001–01) based on the method described in ref. , Y-27632 10.5 μmol/L (3.38 μg/mL; Rho kinase inhibitor; Sigma, #Y0503–5 mg). ..

    Expressing:

    Article Title: Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer
    Article Snippet: Digests were centrifuged and resuspended in 100% Matrigel (Corning #356231). .. Cells were cultured in complete mouse organoid feed media (30) containing Advanced DMEM/F-12 (Thermo Fisher Scientific #12634028), 1 1M HEPES (diluted to 10 mmol/L; Gibco, #15630056), 1X penicillin/streptomycin (100X stock; Thermo Fisher Scientific #15140122), 1XGlutaMAXSupplement (Gibco, #35050061), 0.5 mmol/L (0.21 mg/mL) A83–01 (TGFb inhibitor; Tocris, #2939), recombinant murine EGF (mEGF; 0.05 mg/mL; Gibco, #PMG8041), recombinant FGF (FGF-10; 0.1 mg/mL; Peprotech, #100–26–250 mg), 0.01 mmol/L (0.021 mg/mL) Gastrin I (Tocris, #3006), 0.1 mg/mL, recombinant mNoggin, 1.25 mmol/L (0.2 mg/mL; peprotech #250– 38–250ug), N-acetylcysteine 10mmol/L (1.22mg/mL; Sigma, #A9165– 5g), nicotinamide 10 mmol/L (Sigma, #N0636–100G), 1X B27 supplement (Thermo Fisher Scientific, #17504044), 10% R-spondinconditioned media (produced in-house from a cell line expressing recombinant R-spondin: Trevigen Cat # 3710–001–01) based on the method described in ref. (30), Y-27632 10.5 mmol/L (3.38 mg/ mL; Rho kinase inhibitor; Sigma, #Y0503–5 mg). ..

    Article Title: Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer
    Article Snippet: Digests were centrifuged and resuspended in 100% Matrigel (Corning #356231). .. Cells were cultured in complete mouse organoid feed media ( ) containing Advanced DMEM/F-12 (Thermo Fisher Scientific #12634028), 1×1M HEPES (diluted to 10 mmol/L; Gibco, #15630056), 1X penicillin/streptomycin (100X stock; Thermo Fisher Scientific #15140122), 1X GlutaMAX Supplement (Gibco, #35050061), 0.5 μmol/L (0.21 μg/mL) A83–01 (TGFβ inhibitor; Tocris, #2939), recombinant murine EGF (mEGF; 0.05 μg/mL; Gibco, #PMG8041), recombinant FGF (FGF-10; 0.1 μg/mL; Peprotech, #100–26–250 μg), 0.01 μmol/L (0.021 μg/mL) Gastrin I (Tocris, #3006), 0.1 μg/mL, recombinant mNoggin, 1.25 mmol/L (0.2 mg/mL; peprotech #250–38–250ug), N-acetylcysteine 10 mmol/L (1.22 mg/mL; Sigma, #A9165–5g), nicotinamide 10 mmol/L (Sigma, #N0636–100G), 1X B27 supplement (Thermo Fisher Scientific, #17504044), 10% R-spondin-conditioned media (produced in-house from a cell line expressing recombinant R-spondin: Trevigen Cat # 3710–001–01) based on the method described in ref. , Y-27632 10.5 μmol/L (3.38 μg/mL; Rho kinase inhibitor; Sigma, #Y0503–5 mg). ..



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    Identification of <t>JH015Y</t> protein. (A) Protein electrophoresis map. (B) Mass spectrum. (C) Analysis of JH015Y protein and its matching degree with protein database data. HIVEP3: HIVEP Zinc Finger 3; A member of the human immunodeficiency virus type 1 enhancer binding protein family. NAC-A/B: domains of NAC transcription factors unique to plants. LIM: A highly conserved double zinc finger domains in evolution.
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    Image Search Results


    Identification of JH015Y protein. (A) Protein electrophoresis map. (B) Mass spectrum. (C) Analysis of JH015Y protein and its matching degree with protein database data. HIVEP3: HIVEP Zinc Finger 3; A member of the human immunodeficiency virus type 1 enhancer binding protein family. NAC-A/B: domains of NAC transcription factors unique to plants. LIM: A highly conserved double zinc finger domains in evolution.

    Journal: Biomolecules & Therapeutics

    Article Title: An Amphibians-Derived Protein Provides Novel Biotherapeutics for Various Wounds Treatment

    doi: 10.4062/biomolther.2024.096

    Figure Lengend Snippet: Identification of JH015Y protein. (A) Protein electrophoresis map. (B) Mass spectrum. (C) Analysis of JH015Y protein and its matching degree with protein database data. HIVEP3: HIVEP Zinc Finger 3; A member of the human immunodeficiency virus type 1 enhancer binding protein family. NAC-A/B: domains of NAC transcription factors unique to plants. LIM: A highly conserved double zinc finger domains in evolution.

    Article Snippet: This study utilized thefollowing listed materials:HaCaTcells (maintained in our laboratory); HSF cells (maintained in our laboratory); JH015Y protein; recombinant murine epidermal growth factor (EGF) (PeproTech, 5 Cedarbrook Drive, Cranbury, NJ, USA); DMEM culture medium; fetal bovine serum (Gibco, Carlsbad, CA, USA); penicillin/streptomycin stock solution (Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, 236 Baidi Road, Tianjin, China); CCK-8 assay kit (Servicebio, Wuhan, China); Clean bench (BSC-1100IIA, Beijing Donglian Haer Instrument Manufacturing Co., Ltd., Beijing, China); CO2 cell culture incubator (Shanghai Boxun Medical Biological Instrument Co., Ltd, Shanghai, China); microplate reader (Jiangnan Corporation, Ningbo, China); Depilatory cream (Veet, London, UK); sterile dressing (6×7 cm, Zhejiang Ou Jie Technology Co., Ltd., Deqing, China); glutaraldehyde (JiZhiSheng Biology, Shanghai, China); OCT embedding agent (Tissue-TEK, Sakura, Torrance, CA, USA); Masson’s trichrome staining kit (Nanjing Kaiji Biotechnology Development Co., Ltd., Nanjing, China); cryostat (CM1950, Leica, Wetzlar, Germany); YLS-5Q desktop constant temperature and pressure scald instrument (Tianjin Norei Xinda Technology Co., Ltd., Tianjin, China); blood glucose meter (Sinocare, Changsha, China); Sprague Dawley rats (male, 120-160 g, four-week-old), C57BLKS/J (db/db) mice (male, 40-50 g, six-week-old), provided by Shanghai SLAC Laboratory Animal Co., Ltd., Shanghai, China; BCA protein quantification kit (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); electrophoresis buffer (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); protein molecular weight standards (Shanghai Yaen Biotechnology Co., Ltd., Shanghai, China); sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) gel rapid preparation kit (Dalian Meilun Biotechnology Co., Ltd., Dalian, China); protein loading buffer; chemiluminescence imager (BioRad, Hercules, CA, USA); vertical protein electrophoresis apparatus (Hangzhou Nuoyang Biotechnology Co., Ltd., Hangzhou, China); Maldi TOF mass spectrometer (Bruker, Saarbrucken, Germany); ultra-high resolution mass spectrometer (Thermo Fisher, Waltham, MA, USA).

    Techniques: Protein Electrophoresis, Virus, Binding Assay

    Promoting of JH015Y protein on cell proliferation in vitro . (A) Influence of JH015Y protein in the proliferation of HaCaT cells. (B) Influence of JH015Y protein in the proliferation of HSF cells. *** p <0.001, ** p <0.01, * p <0.05. n=5. EGF: Epidermal Growth Factor. HSF: Human Skin Fibroblasts. HaCaT: Human Keratinocytes Cells.

    Journal: Biomolecules & Therapeutics

    Article Title: An Amphibians-Derived Protein Provides Novel Biotherapeutics for Various Wounds Treatment

    doi: 10.4062/biomolther.2024.096

    Figure Lengend Snippet: Promoting of JH015Y protein on cell proliferation in vitro . (A) Influence of JH015Y protein in the proliferation of HaCaT cells. (B) Influence of JH015Y protein in the proliferation of HSF cells. *** p <0.001, ** p <0.01, * p <0.05. n=5. EGF: Epidermal Growth Factor. HSF: Human Skin Fibroblasts. HaCaT: Human Keratinocytes Cells.

    Article Snippet: This study utilized thefollowing listed materials:HaCaTcells (maintained in our laboratory); HSF cells (maintained in our laboratory); JH015Y protein; recombinant murine epidermal growth factor (EGF) (PeproTech, 5 Cedarbrook Drive, Cranbury, NJ, USA); DMEM culture medium; fetal bovine serum (Gibco, Carlsbad, CA, USA); penicillin/streptomycin stock solution (Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, 236 Baidi Road, Tianjin, China); CCK-8 assay kit (Servicebio, Wuhan, China); Clean bench (BSC-1100IIA, Beijing Donglian Haer Instrument Manufacturing Co., Ltd., Beijing, China); CO2 cell culture incubator (Shanghai Boxun Medical Biological Instrument Co., Ltd, Shanghai, China); microplate reader (Jiangnan Corporation, Ningbo, China); Depilatory cream (Veet, London, UK); sterile dressing (6×7 cm, Zhejiang Ou Jie Technology Co., Ltd., Deqing, China); glutaraldehyde (JiZhiSheng Biology, Shanghai, China); OCT embedding agent (Tissue-TEK, Sakura, Torrance, CA, USA); Masson’s trichrome staining kit (Nanjing Kaiji Biotechnology Development Co., Ltd., Nanjing, China); cryostat (CM1950, Leica, Wetzlar, Germany); YLS-5Q desktop constant temperature and pressure scald instrument (Tianjin Norei Xinda Technology Co., Ltd., Tianjin, China); blood glucose meter (Sinocare, Changsha, China); Sprague Dawley rats (male, 120-160 g, four-week-old), C57BLKS/J (db/db) mice (male, 40-50 g, six-week-old), provided by Shanghai SLAC Laboratory Animal Co., Ltd., Shanghai, China; BCA protein quantification kit (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); electrophoresis buffer (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); protein molecular weight standards (Shanghai Yaen Biotechnology Co., Ltd., Shanghai, China); sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) gel rapid preparation kit (Dalian Meilun Biotechnology Co., Ltd., Dalian, China); protein loading buffer; chemiluminescence imager (BioRad, Hercules, CA, USA); vertical protein electrophoresis apparatus (Hangzhou Nuoyang Biotechnology Co., Ltd., Hangzhou, China); Maldi TOF mass spectrometer (Bruker, Saarbrucken, Germany); ultra-high resolution mass spectrometer (Thermo Fisher, Waltham, MA, USA).

    Techniques: In Vitro

    The promoting effect of JH015Y protein on in vitro cell migration. (A) The effect of JH015Y protein on the migration of HaCaT cells. (B) The effect of JH015Y protein on HSF cell migration. (C) The effect of JH015Y protein on cytokine secretion of HaCaT. (D) The effect of JH015Y protein on cytokine secretion in HSF. *** p <0.001, ** p <0.01, * p <0.05. n=5. HSF: Human Skin Fibroblasts. EGF: Epidermal Growth Factor. VEGF: Vascular Endothelial Growth Factor.

    Journal: Biomolecules & Therapeutics

    Article Title: An Amphibians-Derived Protein Provides Novel Biotherapeutics for Various Wounds Treatment

    doi: 10.4062/biomolther.2024.096

    Figure Lengend Snippet: The promoting effect of JH015Y protein on in vitro cell migration. (A) The effect of JH015Y protein on the migration of HaCaT cells. (B) The effect of JH015Y protein on HSF cell migration. (C) The effect of JH015Y protein on cytokine secretion of HaCaT. (D) The effect of JH015Y protein on cytokine secretion in HSF. *** p <0.001, ** p <0.01, * p <0.05. n=5. HSF: Human Skin Fibroblasts. EGF: Epidermal Growth Factor. VEGF: Vascular Endothelial Growth Factor.

    Article Snippet: This study utilized thefollowing listed materials:HaCaTcells (maintained in our laboratory); HSF cells (maintained in our laboratory); JH015Y protein; recombinant murine epidermal growth factor (EGF) (PeproTech, 5 Cedarbrook Drive, Cranbury, NJ, USA); DMEM culture medium; fetal bovine serum (Gibco, Carlsbad, CA, USA); penicillin/streptomycin stock solution (Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, 236 Baidi Road, Tianjin, China); CCK-8 assay kit (Servicebio, Wuhan, China); Clean bench (BSC-1100IIA, Beijing Donglian Haer Instrument Manufacturing Co., Ltd., Beijing, China); CO2 cell culture incubator (Shanghai Boxun Medical Biological Instrument Co., Ltd, Shanghai, China); microplate reader (Jiangnan Corporation, Ningbo, China); Depilatory cream (Veet, London, UK); sterile dressing (6×7 cm, Zhejiang Ou Jie Technology Co., Ltd., Deqing, China); glutaraldehyde (JiZhiSheng Biology, Shanghai, China); OCT embedding agent (Tissue-TEK, Sakura, Torrance, CA, USA); Masson’s trichrome staining kit (Nanjing Kaiji Biotechnology Development Co., Ltd., Nanjing, China); cryostat (CM1950, Leica, Wetzlar, Germany); YLS-5Q desktop constant temperature and pressure scald instrument (Tianjin Norei Xinda Technology Co., Ltd., Tianjin, China); blood glucose meter (Sinocare, Changsha, China); Sprague Dawley rats (male, 120-160 g, four-week-old), C57BLKS/J (db/db) mice (male, 40-50 g, six-week-old), provided by Shanghai SLAC Laboratory Animal Co., Ltd., Shanghai, China; BCA protein quantification kit (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); electrophoresis buffer (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); protein molecular weight standards (Shanghai Yaen Biotechnology Co., Ltd., Shanghai, China); sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) gel rapid preparation kit (Dalian Meilun Biotechnology Co., Ltd., Dalian, China); protein loading buffer; chemiluminescence imager (BioRad, Hercules, CA, USA); vertical protein electrophoresis apparatus (Hangzhou Nuoyang Biotechnology Co., Ltd., Hangzhou, China); Maldi TOF mass spectrometer (Bruker, Saarbrucken, Germany); ultra-high resolution mass spectrometer (Thermo Fisher, Waltham, MA, USA).

    Techniques: In Vitro, Migration

    Therapeutic effect of JH015Y protein on acute wounds in vivo . (A) The wound healing rate of rats in different groups and days. (B) Photos of rat wounds from different groups on different days. (C) Masson staining results of skin samples from different groups of rats on different days (scale: 100 μm). **** p <0.0001, *** p <0.001, ** p <0.01, * p <0.05. n=8. EGF: Epidermal Growth Factor.

    Journal: Biomolecules & Therapeutics

    Article Title: An Amphibians-Derived Protein Provides Novel Biotherapeutics for Various Wounds Treatment

    doi: 10.4062/biomolther.2024.096

    Figure Lengend Snippet: Therapeutic effect of JH015Y protein on acute wounds in vivo . (A) The wound healing rate of rats in different groups and days. (B) Photos of rat wounds from different groups on different days. (C) Masson staining results of skin samples from different groups of rats on different days (scale: 100 μm). **** p <0.0001, *** p <0.001, ** p <0.01, * p <0.05. n=8. EGF: Epidermal Growth Factor.

    Article Snippet: This study utilized thefollowing listed materials:HaCaTcells (maintained in our laboratory); HSF cells (maintained in our laboratory); JH015Y protein; recombinant murine epidermal growth factor (EGF) (PeproTech, 5 Cedarbrook Drive, Cranbury, NJ, USA); DMEM culture medium; fetal bovine serum (Gibco, Carlsbad, CA, USA); penicillin/streptomycin stock solution (Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, 236 Baidi Road, Tianjin, China); CCK-8 assay kit (Servicebio, Wuhan, China); Clean bench (BSC-1100IIA, Beijing Donglian Haer Instrument Manufacturing Co., Ltd., Beijing, China); CO2 cell culture incubator (Shanghai Boxun Medical Biological Instrument Co., Ltd, Shanghai, China); microplate reader (Jiangnan Corporation, Ningbo, China); Depilatory cream (Veet, London, UK); sterile dressing (6×7 cm, Zhejiang Ou Jie Technology Co., Ltd., Deqing, China); glutaraldehyde (JiZhiSheng Biology, Shanghai, China); OCT embedding agent (Tissue-TEK, Sakura, Torrance, CA, USA); Masson’s trichrome staining kit (Nanjing Kaiji Biotechnology Development Co., Ltd., Nanjing, China); cryostat (CM1950, Leica, Wetzlar, Germany); YLS-5Q desktop constant temperature and pressure scald instrument (Tianjin Norei Xinda Technology Co., Ltd., Tianjin, China); blood glucose meter (Sinocare, Changsha, China); Sprague Dawley rats (male, 120-160 g, four-week-old), C57BLKS/J (db/db) mice (male, 40-50 g, six-week-old), provided by Shanghai SLAC Laboratory Animal Co., Ltd., Shanghai, China; BCA protein quantification kit (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); electrophoresis buffer (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); protein molecular weight standards (Shanghai Yaen Biotechnology Co., Ltd., Shanghai, China); sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) gel rapid preparation kit (Dalian Meilun Biotechnology Co., Ltd., Dalian, China); protein loading buffer; chemiluminescence imager (BioRad, Hercules, CA, USA); vertical protein electrophoresis apparatus (Hangzhou Nuoyang Biotechnology Co., Ltd., Hangzhou, China); Maldi TOF mass spectrometer (Bruker, Saarbrucken, Germany); ultra-high resolution mass spectrometer (Thermo Fisher, Waltham, MA, USA).

    Techniques: In Vivo, Staining

    Therapeutic effect of JH015Y protein on burn wounds in vivo . (A) Photos of rat wounds from different groups on different days. (B) The healing rate of scalds in rats of different groups and days. (C) Masson staining results of skin samples from different groups of rats on different days (scale: 100 μm). *** p <0.001, ** p <0.01, * p <0.05. n=8. EGF: Epidermal Growth Factor.

    Journal: Biomolecules & Therapeutics

    Article Title: An Amphibians-Derived Protein Provides Novel Biotherapeutics for Various Wounds Treatment

    doi: 10.4062/biomolther.2024.096

    Figure Lengend Snippet: Therapeutic effect of JH015Y protein on burn wounds in vivo . (A) Photos of rat wounds from different groups on different days. (B) The healing rate of scalds in rats of different groups and days. (C) Masson staining results of skin samples from different groups of rats on different days (scale: 100 μm). *** p <0.001, ** p <0.01, * p <0.05. n=8. EGF: Epidermal Growth Factor.

    Article Snippet: This study utilized thefollowing listed materials:HaCaTcells (maintained in our laboratory); HSF cells (maintained in our laboratory); JH015Y protein; recombinant murine epidermal growth factor (EGF) (PeproTech, 5 Cedarbrook Drive, Cranbury, NJ, USA); DMEM culture medium; fetal bovine serum (Gibco, Carlsbad, CA, USA); penicillin/streptomycin stock solution (Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, 236 Baidi Road, Tianjin, China); CCK-8 assay kit (Servicebio, Wuhan, China); Clean bench (BSC-1100IIA, Beijing Donglian Haer Instrument Manufacturing Co., Ltd., Beijing, China); CO2 cell culture incubator (Shanghai Boxun Medical Biological Instrument Co., Ltd, Shanghai, China); microplate reader (Jiangnan Corporation, Ningbo, China); Depilatory cream (Veet, London, UK); sterile dressing (6×7 cm, Zhejiang Ou Jie Technology Co., Ltd., Deqing, China); glutaraldehyde (JiZhiSheng Biology, Shanghai, China); OCT embedding agent (Tissue-TEK, Sakura, Torrance, CA, USA); Masson’s trichrome staining kit (Nanjing Kaiji Biotechnology Development Co., Ltd., Nanjing, China); cryostat (CM1950, Leica, Wetzlar, Germany); YLS-5Q desktop constant temperature and pressure scald instrument (Tianjin Norei Xinda Technology Co., Ltd., Tianjin, China); blood glucose meter (Sinocare, Changsha, China); Sprague Dawley rats (male, 120-160 g, four-week-old), C57BLKS/J (db/db) mice (male, 40-50 g, six-week-old), provided by Shanghai SLAC Laboratory Animal Co., Ltd., Shanghai, China; BCA protein quantification kit (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); electrophoresis buffer (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); protein molecular weight standards (Shanghai Yaen Biotechnology Co., Ltd., Shanghai, China); sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) gel rapid preparation kit (Dalian Meilun Biotechnology Co., Ltd., Dalian, China); protein loading buffer; chemiluminescence imager (BioRad, Hercules, CA, USA); vertical protein electrophoresis apparatus (Hangzhou Nuoyang Biotechnology Co., Ltd., Hangzhou, China); Maldi TOF mass spectrometer (Bruker, Saarbrucken, Germany); ultra-high resolution mass spectrometer (Thermo Fisher, Waltham, MA, USA).

    Techniques: In Vivo, Staining

    Therapeutic effect of JH015Y protein on diabetic ulcer in vivo . (A) Photos of mouse wounds from different groups on different days. (B) Healing rate of ulcer wounds in mice of different groups and days. (C) Masson staining results of skin samples from different groups of rats on different days (scale: 100 μm). ** p <0.01, * p <0.05. n=8. EGF: Epidermal Growth Factor.

    Journal: Biomolecules & Therapeutics

    Article Title: An Amphibians-Derived Protein Provides Novel Biotherapeutics for Various Wounds Treatment

    doi: 10.4062/biomolther.2024.096

    Figure Lengend Snippet: Therapeutic effect of JH015Y protein on diabetic ulcer in vivo . (A) Photos of mouse wounds from different groups on different days. (B) Healing rate of ulcer wounds in mice of different groups and days. (C) Masson staining results of skin samples from different groups of rats on different days (scale: 100 μm). ** p <0.01, * p <0.05. n=8. EGF: Epidermal Growth Factor.

    Article Snippet: This study utilized thefollowing listed materials:HaCaTcells (maintained in our laboratory); HSF cells (maintained in our laboratory); JH015Y protein; recombinant murine epidermal growth factor (EGF) (PeproTech, 5 Cedarbrook Drive, Cranbury, NJ, USA); DMEM culture medium; fetal bovine serum (Gibco, Carlsbad, CA, USA); penicillin/streptomycin stock solution (Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, 236 Baidi Road, Tianjin, China); CCK-8 assay kit (Servicebio, Wuhan, China); Clean bench (BSC-1100IIA, Beijing Donglian Haer Instrument Manufacturing Co., Ltd., Beijing, China); CO2 cell culture incubator (Shanghai Boxun Medical Biological Instrument Co., Ltd, Shanghai, China); microplate reader (Jiangnan Corporation, Ningbo, China); Depilatory cream (Veet, London, UK); sterile dressing (6×7 cm, Zhejiang Ou Jie Technology Co., Ltd., Deqing, China); glutaraldehyde (JiZhiSheng Biology, Shanghai, China); OCT embedding agent (Tissue-TEK, Sakura, Torrance, CA, USA); Masson’s trichrome staining kit (Nanjing Kaiji Biotechnology Development Co., Ltd., Nanjing, China); cryostat (CM1950, Leica, Wetzlar, Germany); YLS-5Q desktop constant temperature and pressure scald instrument (Tianjin Norei Xinda Technology Co., Ltd., Tianjin, China); blood glucose meter (Sinocare, Changsha, China); Sprague Dawley rats (male, 120-160 g, four-week-old), C57BLKS/J (db/db) mice (male, 40-50 g, six-week-old), provided by Shanghai SLAC Laboratory Animal Co., Ltd., Shanghai, China; BCA protein quantification kit (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); electrophoresis buffer (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); protein molecular weight standards (Shanghai Yaen Biotechnology Co., Ltd., Shanghai, China); sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) gel rapid preparation kit (Dalian Meilun Biotechnology Co., Ltd., Dalian, China); protein loading buffer; chemiluminescence imager (BioRad, Hercules, CA, USA); vertical protein electrophoresis apparatus (Hangzhou Nuoyang Biotechnology Co., Ltd., Hangzhou, China); Maldi TOF mass spectrometer (Bruker, Saarbrucken, Germany); ultra-high resolution mass spectrometer (Thermo Fisher, Waltham, MA, USA).

    Techniques: In Vivo, Staining

    The therapeutic effects of the JH015Y protein in different wounds.

    Journal: Biomolecules & Therapeutics

    Article Title: An Amphibians-Derived Protein Provides Novel Biotherapeutics for Various Wounds Treatment

    doi: 10.4062/biomolther.2024.096

    Figure Lengend Snippet: The therapeutic effects of the JH015Y protein in different wounds.

    Article Snippet: This study utilized thefollowing listed materials:HaCaTcells (maintained in our laboratory); HSF cells (maintained in our laboratory); JH015Y protein; recombinant murine epidermal growth factor (EGF) (PeproTech, 5 Cedarbrook Drive, Cranbury, NJ, USA); DMEM culture medium; fetal bovine serum (Gibco, Carlsbad, CA, USA); penicillin/streptomycin stock solution (Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, 236 Baidi Road, Tianjin, China); CCK-8 assay kit (Servicebio, Wuhan, China); Clean bench (BSC-1100IIA, Beijing Donglian Haer Instrument Manufacturing Co., Ltd., Beijing, China); CO2 cell culture incubator (Shanghai Boxun Medical Biological Instrument Co., Ltd, Shanghai, China); microplate reader (Jiangnan Corporation, Ningbo, China); Depilatory cream (Veet, London, UK); sterile dressing (6×7 cm, Zhejiang Ou Jie Technology Co., Ltd., Deqing, China); glutaraldehyde (JiZhiSheng Biology, Shanghai, China); OCT embedding agent (Tissue-TEK, Sakura, Torrance, CA, USA); Masson’s trichrome staining kit (Nanjing Kaiji Biotechnology Development Co., Ltd., Nanjing, China); cryostat (CM1950, Leica, Wetzlar, Germany); YLS-5Q desktop constant temperature and pressure scald instrument (Tianjin Norei Xinda Technology Co., Ltd., Tianjin, China); blood glucose meter (Sinocare, Changsha, China); Sprague Dawley rats (male, 120-160 g, four-week-old), C57BLKS/J (db/db) mice (male, 40-50 g, six-week-old), provided by Shanghai SLAC Laboratory Animal Co., Ltd., Shanghai, China; BCA protein quantification kit (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); electrophoresis buffer (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China); protein molecular weight standards (Shanghai Yaen Biotechnology Co., Ltd., Shanghai, China); sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) gel rapid preparation kit (Dalian Meilun Biotechnology Co., Ltd., Dalian, China); protein loading buffer; chemiluminescence imager (BioRad, Hercules, CA, USA); vertical protein electrophoresis apparatus (Hangzhou Nuoyang Biotechnology Co., Ltd., Hangzhou, China); Maldi TOF mass spectrometer (Bruker, Saarbrucken, Germany); ultra-high resolution mass spectrometer (Thermo Fisher, Waltham, MA, USA).

    Techniques: